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Designed for your lab

Simplify workflow and be confident in your allergen testing process. Neogen™ Allergen Testing Products were designed with your needs in mind by a team of experts who use these tests every day. Based on extensive experience with immunoassay technology and testing thousands of unique food samples, they set out to design tests to detect both processed and unprocessed proteins with a simplified workflow. It took years of careful antibody selection and formulation development to create the simplicity and performance provided by these kits. Supported by Neogen scientists and engineers, these test kits give you the reliability and efficiency you have come to expect from Neogen.

Designed to detect processed and unprocessed proteins

With more variety than ever, today’s food products often require complex processing, which can alter the nature of allergenic proteins in food, making them harder to detect. Neogen™ Allergen Testing Products are designed to overcome this challenge by detecting both processed and unprocessed proteins for more predictable, accurate results in your lab.

Versatility across sample types

Neogen™ Allergen Testing Products can be used on environmental swabs, clean-inplace (CIP) final rinse water, ingredients and food samples.

Available Models

The Neogen™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

The Neogen Allergen Protein Rapid Kits utilize a lateral flow device (LFD) that is an immunochromatographic test method utilizing antibodies which are specific for the detection of proteins from specific allergenic foods. Positive results are visualized by the presence of three lines: a control line, a hook line and a test line, when the target protein is present in a defined range of concentration. These limits will vary by a specific protein assay, and the limit of detection may vary depending on the matrix tested. Samples containing high amounts of target protein in a sample may result in an invalid test which will result in the absence of the hook line and/or test line.

  • Results visible at 11 ± 1 minute after application of the sample.
  • Rapid, qualitative immunochromatographic lateral flow test cartridges designed for the detection of allergenic food proteins.
  • Designed to detect both processed and unprocessed allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • Neogen™ Environmental Collection Swabs and Neogen™ Transfer Pipettes sold separately.

Lateral Flow Devices for Specific Allergen Detection

Streamline your process and simplify your allergen testing with Neogen™ Allergen Protein Rapid Tests — qualitative immunochromatographic assays for rapid in-plant monitoring of specific food allergens. Designed for accurate detection of processed and unprocessed allergen proteins, these fast, easy tests can be used for clean-in-place (CIP) final rinse water, environmental swab samples, raw ingredients and finished food products. We’ve taken the hassle out of allergen testing. You see the results.

Lateral Flow Rapid Kits

Allergen Proteins Catalog Number Assay Time Limit of Detection
Almond L25ALM 11 min ± 1 min 2.0 ppm
Milk L25MLK 11 min ± 1 min 3.0 ppm
Cashew L25CHW 11 min ± 1 min 2.0 ppm
Coconut  L25COC 11 min ± 1 min 2.0 ppm
Egg White L25EGG 11 min ± 1 min 0.5 ppm
Fish L25FSH 11 min ± 1 min 1.0 ppm
Gluten  L25GLU 11 min ± 1 min 5.0 ppm
Hazelnut L25HZL 11 min ± 1 min 2.0 ppm
Peanut L25PNT 11 min ± 1 min 1.0 ppm
Pecan L25PEC 11 min ± 1 min 3.0 ppm
Pistachio L25PST 11 min ± 1 min 2.0 ppm
Soy L25SOY 11 min ± 1 min 2.0 ppm
Walnut L25WAL 11 min ± 1 min 2.0 ppm

All kits include 25 lateral flow devices.

Neogen™ Allergen Protein Enzyme-Linked Immunosorbent Assay (ELISA) Tests

Get confirmed, quantifiable results with solid phase sandwich ELISA tests for monitoring specific food allergens across a wide variety of foods. Designed for accurate detection of processed and unprocessed target allergen proteins, this broad portfolio of ELISA assays is one of the most comprehensive available, and can be used for clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products. A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time. It’s the simple way to master allergen testing.

The Neogen™ Allergen Protein ELISA Kits are intended for the detection of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

The Neogen Allergen Protein ELISA Kits utilize a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

  • Designed to detect both processed and unprocessed target allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.
  • Neogen™ Environmental Collection Swabs sold separately.

Elisa Test

Allergen Proteins Catalog Number Assay Time Limit of Detection Limit of Quantification
Almond E96ALM 11 min ± 1 min 1.9 ng/mL (ppb) 1.0 ppm
Bovine Casein E96CAS 50 min 1.7 ng/mL (ppb) 0.5 ppm
Bovine Total Milk E96MLK 50 min 5.8 ng.mL (ppb) 1.0 ppm
Brazil Nut E96BZL 50 min 1.3 ng/mL (ppb) 1.0 ppm
Cashew E96CHW 50 min 2.6 ng/mL (ppb) 0.9 ppm
Coconut E96COC 50 min 9.7 ng/mL (ppb) 2.0 ppm
Crustacean E96CRU 50 min 10.2 ng/mL (ppb) 2.0 ppm
Egg White E96EGG 50 min 2.1 ng/mL (ppb) 0.5 ppm
Fish E96FSH 50 min 2.7 ng/mL (ppb) 1.0 ppm
Hazelnut E96HZL 50 min 1.9 ng/mL (ppb) 1.0 ppm
Macademia E96MAC 50 min 1.1 ng/mL (ppb) 0.3 ppm
Mollusk E96MOL 50 min 7.2 ng/mL (ppb) 1.0 ppm
Mustard E96MUS 50 min 3.1 ng/mL (ppb) 1.5 ppm
Peanut E96PNT 50 min 6.7 ng.mL (ppb) 2.0 ppm
Pecan E96PEC 50 min 3.7 ng/mL (ppb) 0.7 ppm
Pine Nut E96PNE 50 min  1.3 ng/mL (ppb) 0.5 ppm
Pistachio E96PST 50 min 3.5 ng/mL (ppb) 1.0 ppm
Sesame E96SES 50 min 3.7 ng/mL (ppb) 2.0 ppm
Soy E96SOY 50 min 11.0 ng/mL (ppb) 2.0 ppm
Walnut  E96WAL  50 min 6.9 ng/mL (ppb) 2.0 ppm

All kits include one plate of 96 breakaway wells.

  • Cosmetics & Personal Care
  • Education & Academics
  • Food & Beverage
  • Medical
  • Pharmaceutical
NeogenNeogen®’s Solutions Can Be Found at Every Step of the Food Chain Neogen provides a comprehensive range of solutions and services for the food processing, animal protein and agriculture industries, not only helping to protect the world’s food supply, but also enabling our customers to produce more efficiently and effectively than they could before. We are also proud to serve the companion and performance animal industries, as well as the fields of life sciences and toxicology.
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