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Comparison of material specifications

  #1.5 ibidi Polymer Coverslip #1.5H ibidi Glass Coverslip #1.5 glass coverslip Standard glass slide* Standard polystyrene plates & dishes
Bottom thickness 180 µm (+10/–5 µm) 170 µm (+/–5 µm) 170 µm (+20/–10 µm) 1000 µm (+/–50 µm) 500-1000 µm
Bottom material Polymer D 263 M Schott high precision glass D 263 M Schott high precision glass Soda-lime glass Polymer
Surface modifications ibiTreat (tissue culture-treated) Uncoated (hydrophobic) Uncoated glass Uncoated glass Untreated tissue culture-treated Untreated

Applications

  #1.5 ibidi Polymer Coverslip #1.5H ibidi Glass Coverslip #1.5 glass coverslip Standard glass slide* Standard polystyrene plates & dishes
Brightfield Microscopy ++ ++ ++ ++ ++
Phase Contrast ++ ++ ++ ++ ++
Differential Interference Contrast (DIC) ++ ++ ++ ++ -
Widefield Fluorescence ++ ++ ++ ++ -
Confocal Fluorescence ++ ++ ++ ++ -
Two-Photon and Multiphoton Microscopy ++ ++ ++ ++ -
Fluorescence Recovery After Photobleaching (FRAP) ++ ++ ++ ++ -
Förster Resonance Energy Transfer (FRET) ++ ++ ++ ++ -
Fluorescence Lifetime Imaging Microscopy (FLIM) ++ ++ ++ ++ -
Lightsheet Fluorescence Microscopy (LSFM, SPIM) ++ ++ ++ ++ -
Total Internal Reflection Fluorescence (TIRF) + ++ + + -
Super-Resolution Microscopy + ++ + + -

Applications

  #1.5 ibidi Polymer Coverslip #1.5H ibidi Glass Coverslip #1.5 glass coverslip Standard glass slide* Standard polystyrene plates & dishes
Refractive index (nD 589 nm) 1.52 1.52 1.52 1.52 1.58
Abbe number 56 55 55 64 31
Autofluorescence Low Low Low Low Very high
Transmission Very high
(even ultraviolet)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)

Further aspects

  #1.5 ibidi Polymer Coverslip #1.5H ibidi Glass Coverslip #1.5 glass coverslip Standard glass slide* Standard polystyrene plates & dishes
Gas permeability Yes No No No Yes
Material flexibility High Low Low Very low High
Breakable No Yes Yes Yes No
Compatibility with protein coatings Yes Yes Yes Yes Yes
Birefringence (DIC) Low
(DIC-compatible)
Low
(DIC-compatible)
Low
(DIC-compatible)
Low
(DIC-compatible)
Very high
Immersion Oil compatibility Yes Yes Yes Yes No
Correct thickness for high resolution microscopy objective lenses Yes Yes Yes No (Yes, if mounted with a standard glass coverslip) No

 

* With the 3 Well | 8 Well | 12 Well Chamber Slide, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide. They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.