ATTO - Single Live Cell Imaging

Cellgraph is an imaging system designed to detect extremely weak bio- luminescent signals from a single cell. In combination with luciferase markers, Cellgraph can visualize the cell dynamics - time dependent changes of transcriptional activities in individual cells and localization to inner cell components (organelles).

The Cellgraph Viewer software simplifies image analysis with its user-friendly interface. It offers various functions such as luminescent intensity measurement, movie creation, and montage generation. The ROI mode within the analysis tools enables the measurement of bioluminescent intensities in specific regions of interest. The results of this analysis can be exported as CSV format files for further use.

Technical Specifications

Detector	Back- illuminated EM CCD (-80°C) Down to -90°C (water cooling, optional)
Pixels	512 × 512
Objective lens	×4, (optional ×10, 20, 40, 60)
Stage	X-Y-Z axis : Manual Z axis : Motorized (external control)
Sample container	35mm dish
Temperature	room temperature +5°C to 45°C
CO2 (option)	Humidifying Unit, CO2 gas mixer
Lighting	White LED for bright field image Blue LED for GFP excitation
Filter	525(GFP), 560, 620 nm long pass filter
Imaging modes	Live/Interval/Stage control/ Combination/Background
Filter Switch	Automatic control of set filter
Exposure time	30 msec to 90 minutes
Power (main body)	AC 100-240 V 106VA (varies according to the components of the whole system)
Dimension	400 M x 450 (D) x 700 (H) mm
Weight	Approx. 40 kg (Cellaraph main body only)
	View the entire technical specifications in the brochure.

Key Features

ATTO - Single Live Cell Imaging

Real time reporter gene assay
Transcriptional factor activity, Clock gene (chronopharmacology). Gene expression (Transfection,RNAi,etc)
Analysis of cell responses
Drug response(Anti-cancer drug,DDS,etc),Stress response (Hormone,Inflammation,Anti-Oxidant), Cytotoxichology
Analysis of signal pathway
Signal cascade (Calcium), Apoptosis,etc
Acquire images of culturing cells or tissue samples on a 35 mm dish in the living state
Provide a CO₂ incubator equivalent atmosphere by controlling the temperature, CO₂ gas concentration, and humidifying
Highly sensitive and accurate detection of faint bioluminescence with an EM-CCD camera
Isolates and captures multicolor faint bioluminescence
Easy operation via a user-friendly interface
Full control from a PC with dedicated software
Completely lightproof, compact design

Quantitative Data Analysis of Luminescent Intensities in Living Cells

Cellgraph Viewer is an image analysis software. It is very simple and easy to use, and has multiple function such as luminescent intensity measurement, making movie and montage... etc.. The ROI mode in analysis tools provides the function to measure bioluminescent intensities individually in any given region of interest. The result of analyzing bioluminescence intensity data can be exported as CSV format files.

Key Industries

Biopharmaceutical and Biotechnology
Education & Academics
Medical
Pharmaceutical

ATTO Corporation is a scientific instrument developer and manufacturer of protein/nucleic acid research. Long been providing more than 60 years for Polyacrylamide Gel Electrophoresis Devices, PAGEL (precast gel), Western blot solutions, Peristaltic Pumps, Printgraph (High-end Gel Documentation System), Luminograph series (Imaging System for bio/chemiluminescence detection), etc. Their products have been used in many laboratories in Japan and other countries.