CytoSMART - Automated Fluorescence Microscopy

Channels	Brightfield, green and red fluorescent channels
Magnification	10× fixed objective – 20× digital zoom
Fluorescence filters	Green – excitation: 452/45 nm; emission: 512/23 nm Red – excitation: 561/14 nm; emission: 630/90 nm
Camera	6.4 MP CMOS
Light source	LED
Data formats	JPG, TIFF, XLSX, MP4
Image size	2072 × 2072 pixels
Field of view	1.45 mm × 1.45 mm
Culture vessels	Well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels
Computer requirements	Windows 10, 2.4 GHz i5 processor, 4 GB RAM memory, USB 3.0, internet
Dimensions (L × W × H)	166 mm × 140 mm × 135 mm
Weight	1.3 kg
Operating conditions	5 – 40 °C, 20 – 95% humidity
Warranty	1-year
Data storage	Unlimited cloud storage
Extra features	Template functionality (ability to save image acquisition parameters in templates), Object count (count the number of fluorescent objects in the image)

Channels

Brightfield, green and red fluorescent channels

Magnification

10× fixed objective – 20× digital zoom

Fluorescence filters

Green – excitation: 452/45 nm; emission: 512/23 nm
Red – excitation: 561/14 nm; emission: 630/90 nm

Camera

6.4 MP CMOS

Light source

LED

Data formats

JPG, TIFF, XLSX, MP4

Image size

2072 × 2072 pixels

Field of view

1.45 mm × 1.45 mm

Culture vessels

Well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels

Computer requirements

Windows 10, 2.4 GHz i5 processor, 4 GB RAM memory, USB 3.0, internet

Dimensions (L × W × H)

166 mm × 140 mm × 135 mm

Weight

1.3 kg

Operating conditions

5 – 40 °C, 20 – 95% humidity

Warranty

1-year

Data storage

Unlimited cloud storage

Extra features

Template functionality (ability to save image acquisition parameters in templates), Object count (count the number of fluorescent objects in the image)

Key Features

Live insight in cellular processes

Get the whole story and observe the development of cellular processes while they occur. The CytoSMART Lux3 fluorescence imaging microscope automatically creates time-lapse movies that contain many cellular features. The videos are made from inside the incubator without disturbing your cells and can be immediately accessed and analyzed remotely via the CytoSMART Cloud, providing real-time updates on your cell cultures and running experiments.

NEW - Quantification of real-time cellular events

For certain experiments, in addition to monitoring cell cultures, it is also required to quantitatively evaluate the progression of cellular events. The CytoSMART Lux3 FL, equipped with a new Object Count algorithm, can automatically calculate the number of fluorescent objects in the image and investigate how this number changes over time. What’s more, the existing software interface has become even more intuitive, allowing users to quickly and easily adjust the parameters of an image.

Analyze cells in their desired culture environment

Kinetic fluorescence imaging of cellular processes requires environmental control throughout the experiment. Because of its small size, the Lux3 FL fits inside any standard incubator. This enables you to perform fluorescent live-cell imaging experiments parallel to your regular cell cultures. Because of the remote data accessibility in the CytoSMART Cloud, your cells do not have to be disturbed during the experiment.

Simultaneous analysis of multiple cell features

Using CytoSMART Lux3 FL, you can analyze green and red fluorescent tags simultaneously with your brightfield images. This not only saves you time but also provides tools to distinguish cells from each other or distinguish labeled structures within the cells. By tracking changes in the fluorescent signal over time, cell number changes or fluorescently tagged cellular components can easily be identified.