Ibidi - Labware - µ-Slide Membrane ibiPore Flow

Shown here is a time lapse movie of a transmigrating human HL-60 cell that was seeded into the lower channel of a µ-Slide Membrane ibiPore Flow (pore size 3 µm). Under flow conditions, the cell transmigrates in vitro through the porous glass membrane into the upper channel that was filled with a 3D collagen matrix containing the chemoattractant fMLP.

Live cell imaging of a transmigrating single SiR-Actin-stained cell (indicated by pseudo colors) by spinning disc confocal microscopy (20x objective) with a time interval of 30 s. The arrow indicates the direction of flow at a shear stress of 1 dyne/cm2; Scale bar = 10 um. Courtesy R. Pick, Munich, Germany.

• SiMPore’s G-FLAT™ Microporous Glass Membranes
• Cross-channel structure with a porous optical membrane in between
• Excellent optical properties comparable to a glass coverslip
• Pore sizes 0.5 µm, 3 µm, 5 µm, or 8 µm available
• Membrane thickness 0.3 µm (300 nm)
• For use with objective lenses with a working distance >0.5 mm
• Fully compatible with the ibidi Pump System
• Defined shear stress and shear rate levels