Corning - Supplements and Reagents - Cell Dissociation Reagents

Available Products

100 mL Accutase® Cell Detachment Solution, Liquid

Accutase is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including our selection of Advanced Surface coated plasticware.

Efficient, with gentle detachment of adherent cells within minutes
An alternative to Trypsin
Able to eliminate neutralization or wash steps
Accutase does not contain mammalian or bacterial derived products.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that is FDA registered. This product is produced under high quality industry standards.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-058-CI	100 mL Accutase® Cell Detachment Solution, Liquid	1 / Pk	1 / Ea	Corning®	per each

Corning® Polysucrose 400, Powder

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
61-196-RM	Corning® 100 g Polysucrose 400, Powder	1 / Pk	1 / Ea	Corning®	per each
61-196-RO	Corning® 500 g Polysucrose 400, Powder	1 / Pk	1 / Ea	Corning®	per each

Corning® 250 mL DMSO (Dimethyl Sulfoxide)

Dimethyl Sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.

Complete Certificate of Analysis available for each production lot, along with full formulation information Manufactured in a facility that complies with ISO 13485 certified quality system. This product is produced under high quality industry standards.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-950-CQC	Corning® 250 mL DMSO (Dimethyl Sulfoxide)	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL Trypsin 10x, 2.5% Trypsin in HBSS [-] calcium, magnesium, phenol red, Porcine Parvovirus Tested

Trypsin is an enzymatic reagent that is used to release adherent cells from tissue culture plates for passaging. This offering contains trypsin at a concentration of 2.5%, a tenfold concentration of a conventional working volume.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-054-CI	Corning® 100 mL Trypsin 10x, 2.5% Trypsin in HBSS [-] calcium, magnesium, phenol red, Porcine Parvovirus Tested	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL Trypsin 1X, 0.25% Trypsin in HBSS [-] calcium, magnesium, Porcine Parvovirus tested

Trypsin is an enzymatic reagent that is used to release adherent cells from tissue plates for passaging.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-050-CI	Corning® 100 mL Trypsin 1X, 0.25% Trypsin in HBSS [-] calcium, magnesium, Porcine Parvovirus tested	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL Trypsin EDTA 1X

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-053-CI	Corning® 100 mL Trypsin EDTA 1X	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5

0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) Technical Advantage: Can be used for a variety cell counting methods requiring information on cell count and viability. Trypan Blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan blue can be used in assays providing total cell count, viable cell or nonviable cell count alone. This solution is provided at the common concentration of 0.4% (w/v).

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-900-CI	Corning® 100 mL Trypan Blue Solution, 0.4% (w/v) in PBS, pH 7.5 ± 0.5	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL Trypsin EDTA 1X, 0.05% Trypsin/0.53 mM EDTA in HBSS

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-051-CI	Corning® 100 mL Trypsin EDTA 1X, 0.05% Trypsin/0.53 mM EDTA in HBSS	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL HT (Hypoxanthine, Thymidine), 50x

Hypoxanthine and Thymidine (HT) media is a "rescue media" that provides pre-formed purines and pyrimidines to overcome the effects of residual intracellular Aminopterin.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-047-CI	Corning® 100 mL HT (Hypoxanthine, Thymidine), 50x	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL HAT (Hypoxanthine, Aminopterin, Thymidine), 50x

For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.

HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells. The combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor, with hyproxanthine and thymidine, which are intermediates in DNA synthesis, provides a form of artificial selection for cells containing functioning hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and thymidine kinase (TK). These enzymes are involved in DNA synthesis and cell division. HAT medium is often used in the preparation of monoclonal antibodies.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-046-CI	Corning® 100 mL HAT (Hypoxanthine, Aminopterin, Thymidine), 50x	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL Trace Elements B, 1000x

Trace Elements B Solution has the same composition as Cleveland's Trace Element I. The combination of Trace Elements B contains the trace elements of MCDB 104 and of MCDB 301.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-022-CI	Corning® 100 mL Trace Elements B, 1000x	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL Sodium Pyruvate, Liquid 100 mM Solution [+] 8.5 g/L NaCI

100 mM Solution with 8.5 g/L NaCI Sodium pyruvate is often used as a component in culture broth and media as an energy source for propagating cells. This reagent is also used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid. It is provided as a 100 mM Solution with 8.5 g/L NaCl.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-000-CI	Corning® 100 mL Sodium Pyruvate, Liquid 100 mM Solution [+] 8.5 g/L NaCI	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL Cellstripper™, Liquid

Exceptionally gentle on cells compared with traditional enzymatic cell dissociation reagents Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper achieves superior results in comparison to enzymatic dissociation products.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-056-CI	Corning® 100 mL Cellstripper™, Liquid	6 / Pk	6 / Cs	Corning®	per case

Corning® 100 mL Trace Elements C, 1000x

Mediatech's Trace Elements C Solution has the same composition as Cleveland's Trace Elements I, and contains the trace elements found in the serum-free medium MCDB301.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-023-CI	Corning® 100 mL Trace Elements C, 1000x	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL 45% glucose solution

Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media. This 45% (w/v) solution has been to ensure no cytotoxicity.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-037-CI	Corning® 100 mL 45% glucose solution	1 / Pk	1 / Ea	Corning®	per each

Corning® Trypsin

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-052-CI	Corning® 100 mL Trypsin EDTA 1X	6 / Pk	6 / Cs	Corning®	per case
25-052-CV	Corning® 500 mL Trypsin EDTA 1X	6 / Pk	6 / Cs	Corning®	per case

Corning® LSM (Lymphocyte Separation Medium)

Lymphocyte Separation Medium was originally designed for the in vitro isolation of lymphocytes from diluted whole blood. It is a sterile filtered, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/cm³ at 20°C.

Product Number	Product Name	Qty./Pk	Qty./Cs	Brand	Unit Size
25-072-CI	Corning® 100 mL LSM (Lymphocyte Separation Medium)	1 / Pk	1 / Ea	Corning®	per each
25-072-CV	Corning® 500 mL LSM (Lymphocyte Separation Medium)	1 / Pk	1 / Ea	Corning®	per each

Corning® 100 mL Trace Elements A, 1000x

Trace Elements A provides copper, zinc, iron, and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB 301.