Ibidi - Labware - µ-Slide 8 Well high

• Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
• ibiTreat (tissue culture-treated) surface for optimal cell adhesion
• Imaging chamber slide with excellent optical quality for high-end microscopy
• Individual well walls for minimizing well-to-well crosstalk and contaminations
• Compatible with staining and fixation solutions
• Biocompatible polymer material—no glue, no leaking
• Also available as an adhesive version without a bottom: sticky-Slide 8 Well high
• Also available with a Glass Coverslip Bottom: µ-Slide 8 Well high Glass Bottom for special microscopic applications
• Additional version available with a 500 µm grid: µ-Slide 8 Well high Grid-500

• The Coverslip Bottom

  The µ-Slide 8 Well high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 8 Well high without any bottom and the µ-Slide 8 Well high Glass Bottom for special microscopic applications.

• The ibiTreat Surface

  ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Immunofluorescence

The ibidi µ-Slide 8 Well high allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.

Fluorescence microscopy of HuH7 cells after mRNA transfection with eGFP (green). Actin cytoskeleton (Phalloidin, yellow), nuclei (DAPI, blue), mRNA (FISH,Quasar 670, red), 40x objective lens, oil immersion.

The µ-Slide 8 Well high enables high-resolution live cell imaging using different microscopy techniques. 

Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.

Time-lapse phase contrast microscopy of rat fibroblast cells using the µ-Slide 8 Well high ibiTreat in combination with the ibidi Heating System and the ibidi Gas Incubation System. 63x objective lens, oil immersion, 3 min image intervals for 23 hours.