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Ibidi - Instruments - ibidi Pump System

A pump system for the cultivation of, e.g. endothelial, cells under flow for simulation of blood vessels

Product outline:

  • A pump system for the cultivation of, e.g. endothelial, cells under flow for simulation of blood vessels.
  • Ideal simulation of various physiological conditions - continuous unidirectional, oscillating, and pulsatile flow.
  • Compatible with the ibidi Heating Systems, all incubators, incubated microscopes, and Channel µ-Slides.
  • Minimal mechanical stress, minimal amount of medium and supplement needed.

Technical features

  • Operates up to four parallel Fluidic Units per ibidi Pump
  • Flow characteristics: all flow types are laminar
  • Some examples:
  • Unidirectional flow simulates veins or small arteries
  • Oscillating flow simulates disturbed conditions at vessel branching’s or valves
  • Pulsatile flow simulates arterial conditions
  • Wide range of flow rates (ca. 0.1–40 ml/min*) and shear stress (ca. 0.2–150 dyn/cm2*)
  • Working volume: 2.5 ml / 12 ml / 50 ml
  • Suitable for all µ-Slides with Luer adapters
  • Also suitable for home-made flow chambers
  • Compatible with all incubators
  • Software-controlled flow rates and shear stress

*Depending on the Perfusion Set and Channel used.

System overview

Applications

  • Defined shear stress in long-term culture (e.g., endothelial cells, kidney, or biofilm)
  • Live cell imaging and immunofluorescence for analysing shear stress response
  • Mimicking shear stress conditions in microcapillary, venous, and arterial flow
  • Rolling and adhesion of suspended cells on substrates.
  • Stop flow experiments
  • 3D cell culture: interstitial flow

Components of the ibidi Pump System

ibidi Pump

  • Computer-controlled air pressure pump
  • Operates up to four parallel Fluidic Units per ibidi Pump

PumpControl Software

  • Easy setup of an automated flow control for flow assays
  • Internal calculation for flow rates, shear rates, and shear stress

Perfusion Set

  • Includes tubing and reservoirs to perform flow assays in combination with the Fluidic Unit
  • Designed for various flow rates and volumes

Fluidic Unit

  • Flow-switching device with electrically controlled valves
  • Equipped with a holder for one Perfusion Set (10 ml) and connected µ-Slide(s)
  • For operation in the cell culture incubator
  • Optional: Reservoir Holder for 2 ml and 50 ml Perfusion Sets

Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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