Display Type
|
16-character liquid crystal LCD backlit
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---|---|
Power Source Voltage
|
100 to 240 VAC, 50 to 60 Hz, CAT I
|
Operating Temperature
|
10°C to 40°C
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Cooling
|
Convection through metal case
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Relative Humidity
|
60%
|
Pollution Degree
|
Not to be operated in conductive pollutants atmosphere
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Altitude
|
2,000 m (operating)
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The ECM 399 is an exponential decay wave electroporaton generator specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM 399 has a limited capability for transfecting some mammalian cell lines.
The ECM 399 is ideal for basic transformation in research and academic environments.
The ECM 399 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
User Interface
|
Touch Screen Display, Footswitch
|
---|---|
Voltage Range
|
50 to 1,000 Volts
|
Pulse Width Range
|
0.050 to 10 ms
|
Pulse Interval
|
0.200 to 1,000 ms (5 kHz to 1 Hz)
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Pulse Amplitude
|
50 to 1,200 Volts
|
Data Export
|
USB Flash Drive
|
Dimensions (W x H x D)
|
32 cm x 20 cm x 40 cm
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(12.6 x 7.9 x 15.7 in)
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(12.6 x 7.9 x 15.7 in)
|
Weight
|
25 lb (11.3 kg)
|
The AgilePulse MAX System is an advanced electroporation solution for fast, efficient transfection of up to 10 ml of cell suspension. Specifically engineered for large-volume applications, our system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.
The AgilePulse MAX System is simple to use. Cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation Medium T or Cytoporation Medium T4 and transferred via sterile syringe to the large volume electroporation chamber where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes. These are followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented PulseAgile technology optimizes these pulse parameters to maximize efficiency and cell viability.
The system includes a user-friendly, programmable waveform generator with patented PulseAgile technology, our patent-pending large-volume electroporation chamber, and proprietary BTXpress Cytoporation Medium T, optimized for large volume electroporation. The system is engineered to provide uniform electric fields in a stable temperature environment for excellent cell viability.
Model
|
GEMINI X2
|
---|---|
Voltage Range: LV Mode
|
5 to 500 V in 1 V steps
|
Voltage Range: HV Mode
|
505 to 3000 V in 5 V steps
|
Voltage Accuracy
|
5%
|
Pulse Length Range: LV Mode
|
10 μs to 999 μs in 1 μs steps
1 ms to 999 ms in 1 ms steps
|
Pulse Length Range: HV Mode
|
10 μs to 600 μs in 1 μs steps
|
The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.
These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.
Display Type
|
7 inch color touchscreen interface
|
---|---|
Power Source Voltage
|
100 to 240 VAC, 50 to 60 Hz, CAT II Power 350 W (Pulsing), 35 W (Idle)
|
Fusing
|
Type 3AG, 5 A, 250 V 1/4 x 1 1/4 in; Type T (time delay)
|
Intended Use
|
Indoor use only
|
Operating Temperature
|
4° C to 40° C
|
Cooling
|
Convection through metal case
|
Relative Humidity
|
20% to 80% RH, non-condensing
|
Electroporation system primarily used for bacteria and yeast transformation. High throughput (25- and 96-well) models available.
The ECM 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. The ability to select the resistance and capacitance values, and adjust the range of voltages is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection. The ECM 630 can perform the widest range of electroporation applications among commercially available electroporation generators.
Flexibility is important, so BTX has designed a plug and play system for our ECM 630 system to transition between standard cuvettes and to 25/96-well plates using High Throughput Plate Handlers.
Legacy ECM 630 Systems are non RoHS compliant, and thus not available for purchase in the European Union. While supplies last, Legacy ECM 630 systems are available to customers in the Americas and Asia as we transition to new ECM 630 instruments.
The ECM 630 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
Display Type
|
7 inch color touchscreen
|
---|---|
Power Source Voltage
|
100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
|
Fusing
|
Type 3AG, 5 A, 250 V; Type T (time delay)
|
Intended Use
|
Indoor use only
|
Operating Temperature
|
10°C to 40°C
|
Cooling
|
Convection through metal case
|
Relative Humidity
|
60%
|
Altitude
|
2,000 m (operating)
|
The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer.
The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.
The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.
The ECM 830 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
Frequency
|
1 MHz
|
---|---|
Voltage
|
0 - 75 Vrms
|
Duration
|
0 - 99 sec
|
Post fusion AC
|
1/10 of pre-fusion amplitude
|
Post fusion ramp
|
1 - 9 sec
|
Pause between AC/DC
|
50 µsec
|
The Legacy ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.
The LEGACY ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.
The Legacy ECM 2001 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
User Interface
|
Touch Screen Display, Footswitch
|
---|---|
Voltage Range
|
50 to 1,000 volts
|
Pulse Width Range
|
0.050 to 10 ms
|
Pulse Interval
|
0.200 to 1,000 ms (5 kHz to 1 Hz)
|
Data Export
|
USB Flash Drive
|
Dimensions (W x H x D)
|
Dimensions (W x H x D)
|
(with handle)
|
32 cm x 20 cm x 40 cm
|
(12.6 in x 7.9 in x 15.7 in)
|
(12.6 in x 7.9 in x 15.7 in)
|
Weight
|
25 lb (11.3 kg)
|
Operating Temperature
|
10 to 40oC
|
Mains Voltage
|
100 to 250 VAC
|
Fuse
|
5 Amp Slo-Blo, 5 mm x 20 mm
|
Software
|
IM (Intra-muscular) or ID (Intra-dermal)
|
The AgilePulse In Vivo System, used for animal immunization, vaccine development, and gene therapy, provides an intra-muscular, intra-dermal, or intra-tumor electroporation solution to produce maximum transfection efficiency.
Traditional in vivo nucleic acid delivery systems, such as gene gun delivery, suffer from poor efficiency. The AgilePulse In Vivo System effectively introduces DNA or RNA into the target tissue, representing a powerful and safe means for stimulating an immune response that recognizes and eliminates target molecules in the body.
For vaccine applications, DNA vaccination through the dermal layer is preferred since it is an easily accessible site that is immunologically active. After direct injection of plasmid DNA in the dermal layer, a programmed sequence of electric pulses is applied through a miniature parallel needle electrode array to promote cellular uptake and transfection. Cells in the surrounding tissue are transfected, including dendritic antigen-presenting cells and mesenchymal origin cells. Gene expression simulates the immune system to respond to the secreted antigen. Gene expression in skin is 100-fold higher when delivery is enhanced by electroporation compared to simply injecting plasmid DNA (Roos, et. al. 2009).
Voltage Range: LV Mode
|
5 to 500 V in 1 V steps
|
---|---|
Voltage Range: HV Mode
|
505 to 3000 V in 1 V steps
|
Voltage Accuracy
|
5%
|
Pulse Length Range: LV Mode
|
10 to 999 µs in 1 µs steps
|
Pulse Length Range: HV Mode
|
10 to 600 µs in 1 µs steps
|
The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.
The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.
BTX has been at the forefront of electroporation technology since we introduced the first commercially available electroporator in 1983. For over 30 years, we have made it our priority to focus only on electroporation and electrofusion. This focus has allowed us to develop the experience and expertise to supply you with a broad selection of innovative in vivo and in vitro tools to advance your research.
Whatever your need, our range of electroporation systems and accessories covers virtually every electroporation and electrofusion application, including high throughput. Additionally BTX provides exceptional applications support and service before, during and after your purchase to ensure your success.