Solida Biotech Bioprocess Analysers

OUR ANALYSERS

Offer a simple, fast and flexible method for determination of key parameters in fermentation broths, cell culture media and other microbial applications.

• BIO-GM8 industrial multi assay analyser, 11 assay test menu
• BIO-GL6 industrial multi assay analyser, 5 assay test menu
• BIO-GM10 industrial gloucose analyzer, 3 assays.

BIO-GM8 INDUSTRIAL MULTI ASSAY ANALYSER
Assays include: glucose, lactate, ethanol, methanol, ammonia, glycerol, sucrose, lactose, glutamine and NAD / NADH.

BIO-GL6 INDUSTRIAL MULTI ASSAY ANALYSER
Assays include: glucose, lactate, ethanol, methanol and glycerol.

IO-GM10 INDUSTRIAL GLUCOSE ANALYSER
Assays include: glucose.

The CO2 / O2 gas sensor analyses the amount of oxygen uptake and carbon dioxide production in the outlet gas from your bioreactor or fermenter. Real-time monitoring of CO2 and O2 is a valuable tool for the measurement of cell growth within your fermenter vessels. Monitoring these changes can improve productivity by indicating the correct rates for oxygen flow and substrate feed. Using our gas analyser you can also calculate respiratory quotient (RQ), oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and metabolic rate.

SOLIDA infrared gas analysers can be used to simultaneously measure the concentration of CH4 and H2 in sample gases. This is based on the single source two beams non dispersion infrared (NDIR) method for CH4, CO2, and the fuel cell method (ECD) for H2S and O2.
The general applications are landfill, anaerobic digestion and other fermentation processes.

On-line measurement of total cell density

The Solida OD-CELL sensor performs online measurement of total cell density in solution. The sensor is based on optical density, which measures the turbidity of the cell suspension. The measurement is made at NIR (near-infrared) wavelengths so it is insensitive to changes in media colour.

All particles and molecules that absorb or scatter light at 880 nm will be detected, including living and dead cells and cell debris. This measurement is effective after inoculation, when cells are expanding quickly but concentrations are low, making permittivity-based readings less reliable.

Simple online measurement of cell growth
Reliable values during the growth phase
Early detection of process deviations

The Solida Biotech stand-alone methanol sensor provides reliable, online control of methanol concentration in cultures of pichia pastoris or other methylotrophic organisms, allowing fast and efficient optimisation of recombinant protein production. Accurate regulation of methanol concentration is necessary to maintain induction and prevent the accumulation of methanol to cytotoxic levels.
The dissolved oxygen spike method has been used to estimate the methanol concentration. However, this technique exposes the cells to potentially non-inducing levels of methanol and cannot easily be implemented with Mut- clones or with mixed substrate feeds.
Solida Biotech Methanol Sensor overcomes these limitations, giving accurate monitoring and control of methanol concentration in cultures of Mut+, Mut- and MutS recombinant pichia pastoris clones.