Imaging live cells and specimens requires a “gentle” imaging technique which preserves physiological conditions and specimen integrity. Confocal microscopes integrate high-performance detectors and cameras, specialized illumination systems, imaging software and other dedicated components to make a live-cell imaging solution that meets the needs of scientists.

Cancer is a complex and heterogeneous disease caused by cells deficient in growth regulation, disrupting normal function and resulting in uncontrolled cell growth and proliferation. Confocal microscopes help researchers reveal insights into the mechanisms of cancer at the subcellular and molecular levels.

In order to better understand the nervous system, neuroscientists often investigate complex specimens using confocal microscopy to overcome challenges. 

Fluorescence lifetime imaging provides additional information that can help you improve the quality of your confocal imaging. It can be particularly useful to discriminate fluorescence probes that have overlapping fluorescent emission spectra or to eliminate unwanted background fluorescent signals.

FLIM is a crucial application for measuring and quantifying imaging data. As lifetime information is independent of fluorophore concentration, it is extremely well suited for functional imaging. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species and enables further investigation of molecular function, interactions, and their environment. super-resolution in three dimensions. Underlining the impact of super-resolution.