How Advanced Testing and Analysis Is Helping to Curb the Threats of Mycotoxin in Asia Our expertise

In Southeast Asia, the mycotoxin risk is considered extreme as the production and the demand for raw materials used in animal feed are rising every year. According to the Biomin Mycotoxin Survey, the most prevalent mycotoxins in the region during the first half of 2020 were fumonisin, aflatoxin, Don, and zearalenone, which were found over the maximum recommended levels in more than 70 percent of the samples tested in the survey.

As Asia relies substantially on the livestock and aquaculture sectors as economic pillars, having an effective mycotoxin prevention strategy in place is of utmost importance for key stakeholders such as nutritionists, veterinarians, researchers, and raw material handlers across the agro-food chain.

This has led to the growing mycotoxin testing market in Asia. Some of the major factors which have led to the rapid rise for food testing and particularly, mycotoxin testing are:

• Increasing need for safety and consumer awareness of mycotoxins
• Strengthening government regulations on food safety
• Lack of food control infrastructure and instrumentation in emerging markets
• Technological advancements for rapid testing and detection

There are many methods to determine the presence of mycotoxins in a variety of foods and feeds. Besides performance, other key criteria include the speed of analysis, level of technical skills required to perform the assay, and whether the assay provides a qualitative or quantitative result. Below are some of the methods used.

Confirmatory methods:

• Thin-layer chromatography
• Gas chromatography
• High-performance liquid chromatography
• Liquid chromatography-mass spectrometry

Rapid screening methods:

• Enzyme-linked immunosorbent assays
• Dipsticks and lateral flow tests
• Fluorescence polarization immunoassays

While there is increasing demand for rapid and simple methods which are easier to use in the field, there is also a growing preference for more reliable reference methods which can detect the largest possible number of toxins within a single analysis. These include:

• Multi-toxin (multiplex) flow tests
• Molecularly imprinted polymers and aptamers
• Rapid spectroscopy methods
• Biosensors
• Bead-based assays
• High-resolution mass spectrometry

Apart from this, there is also a demand for extraction methods that require less harmful organic extraction solvents without a loss of quality of analysis. As detection technologies continue to advance, we will continue to witness more improvements around mycotoxin analysis. While some are in their advanced stages of development, others still need to overcome the hurdles in making the transition from proof-of-concept assays using toxins in buffer solutions, to the analysis of real food samples.