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Western blotting is the basic experimental method to specifically and high-sensitively detect desired component from protein sample. It is roughly divided into 2 types; "Semi Dry type" and "Wet type (Tank type)". ATTO, recommend and provide Semi Dry type blotting system based on the following merits.

  • Less Amount of Blotting Solution
  • Excellent Operability
  • Small Heating Value
  • Short Operating Time
  • More Effective With Optimized Blotting Solution

Technical Specifications

Model WSE-4115

WSE-4125

WSE-4025

WSE-4045

Electrode size 95 (W) x 98 (D) mm 205 (W) x 100 (D) mm 205mm(W)×100mm(D) 205 (W) x 200 (D) mm
Distance between electrodes Min: 3 mm. Max: 10 mm Min: 3 mm, Max: 15 mm Min: 3 mm, Max: 15 mm Min: 3 mm. Max: 15 mm
Available number of gel for blotting 1 mini gel 2 mini gels Maximum of 2 mini gels 4 mini gels
Method Vertical (Semi-dry blotting) Vertical (Semi-dry blotting) Horizontal (semi-dry blotting) Vertical (Semi-dry blotting)
Blotting solution volume 50-100 mL (depending on the size and number of gel) 100-200 mL (depending on the size and number of gel) 100-200 mL (depending on the size and number of gel) 100-400 mL (depending on the size and number of gel)
Electrode polarity Top: Cathode / Bottom: Anode Top: Cathode / Bottom: Anode Top: Cathode / Bottom: Anode Top: Cathode / Bottom: Anode
Power supply STD mode: 12V constant voltage
RAPID mode: 24V constant voltage
Timer: 1-200 min (display the remaining time)
STD mode: 12V constant voltage
RAPID mode: 24V constant voltage
Timer: 1-250 min (display the remaining time)
Power consumption: 26W
AC adapter Input: 100-240V (#10%) 50/60Hz (‡5%)
Outout: DC24V/1.5A
Input: 100-240V (#10%) 50/60HZ (‡5%)
Output: DC24V/1.5A
Material Main unit: Acrylic
Anode: Platinized titanium
Cathode: Corrosion-resistant stainless steel
Power supply case: ABS resin
Main unit: Acrylic
Anode: Platinized titanium
Cathode: Corrosion-resistant stainless steel
Main Body: Acrylic
Upper Electrode: Chemical-resistant Stainless Steel
Lower: Platinum-plated Titanium
Main unit: Acrylic
Anode: Platinized titanium
Cathode: Corrosion-resistant stainless steel
Dimension / Weight Main unit (without projection): 170 (W) x 120 (D) x 107 (H) mm, 0.77 kg
AC adapter(without projection): 36 (W) x 95 (D) x 31 (H) mm, 0.14 kg
Main unit (without projection): 246 (W) x 186 (D) x 114 (H) mm, 2.1 kg
AC adapter(without projection): 36 (W) x 95 (D) x 31 (H) mm, 0.14 kg
246mm (W) x 145mm (D) x 92mm (H) (excluding protrusions)
1.8kg (excluding lead wire)
246 (W) x 235 (D) x 92 (H) mm (Main unit without projection)
3.0 kg (except wiring)
Components WSE-4115 main unit 1 ea
AC adapter 1 ea
Extension cable 1 ea
Power lead wire with safety terminal and reverse polarity prevention 1 ea
Roller 1 ea
WSE-4125 main unit 1 ea
AC adapter 1 ea
Extension cable 1 ea
Power lead wire with safety terminal and reverse polarity prevention 1 ea
Roller ea
WSE-4025 Main Body: 1 unit
Lead Wire with Reverse Connection Prevention Mechanism and Safety Terminal: 1 piece
Roller Fixture: 1 piece
Instruction Manual: 1 booklet
WSE-4045 main unit 1 ea
Power lead wire with safety terminal and reverse polarity prevention 1 ea
Roller 1 ea

View the entire technical specifications in the brochure.

Available models

For use in 1 gel of compact size/ mini gel size

Key Features

  • Semi-dry blotting system built-in power supply part

  • Save space

  • Output mode can be selected from 2 modes

1. STD (Standard):
Constant voltage 12 V
Standard transferring time:30-60 min (Timer: 1-200 min)
2. RAPID:
Constant voltage 24 V
Standard transferring time:10-30 min (Timer: 1-200 min)

  • Easily and certainly setting membrane, gel and filter

  • Upper electrode includes springs so clear blotting is possible

For use in 2 gels of mini gel size

Key Features

  • Semi-dry blotting system with a built-in power supply offering;
    Fast blotting is available, space-saving, easy operation, high reproducibility

  • Improved Electrode plate setting enabled;
    Uniform and firm contact of gels and membranes, and fast blotting

  • Electrode plate size: 205mm x 100mm

  • Two mini gels can be transferred simultaneously 

  • It takes 10min minimum in fast blotting mode, and 30 to 60min in standard blotting mode

  • Including a hand roller for setting gels/membranes/filter papers

  • About 1/10 of transfer buffer of wet type (tank) is just enough

  • Chemically resistant electrode plate can be washed after use to be kept clean

Semi-dry blotting for transferring proteins from 2 mini gels

Key Features

  • Improved Electrode plate setting enabled;
    uniform and firm contact of gels and membranes; fast blotting

  • Electrode plate size: 205 mm X 100 mm

  • For use in 2 mini gels

  • Uniform transfer efficiency with improved contact of gels and membranes

  • Available for standard and fast blotting system
    Standard blotting condition: Constant voltage, 12 V, 30-60 min
    Fast blotting condition: Constant voltage, 24 V, 10 min

  • Rapid semi-dry blotting is possible with AE-1465 EzFastBlot (10-30min)

  • Blotting high molecular protein is possible with WSE-7210 EzFastBlot HMW (30-60min)

  • Including a hand roller for setting gels/membranes/filter papers

  • About 1/10 of transfer buffer of wet type (tank) is just enough

  • Chemically resistant electrode plate can be washed after use to be kept clean

Semi-dry blotting for transferring proteins from 2 mini gels

Key Features

  • Improved Electrode plate setting enabled;
  • uniform and firm contact of gels and membranes; fast blotting
  • Electrode plate size: 205×200 mm
  • For use in 4 mini gels (8 x 9cm), or Larger size gel blotting is available
  • Rapid semi-dry blotting is possible with AE-1465 EzFastBlot (10-30min)
  • Blotting high molecular protein is possible with WSE-7210 EzFastBlot HMW (30-60min)
  • Expert roller included for easy setting layout (gel, membrane, filter paper)
  • 1/10 of transfer buffer is enough of a wet blot
  • Electrode for drug resistance: washable system

Western Blotting Operation Video

  • Biopharmaceutical and Biotechnology
  • Education & Academics
  • Medical
  • Pharmaceutical
ATTOATTO Corporation is a scientific instrument developer and manufacturer of protein/nucleic acid research. Long been providing more than 60 years for Polyacrylamide Gel Electrophoresis Devices, PAGEL (precast gel), Western blot solutions, Peristaltic Pumps, Printgraph (High-end Gel Documentation System), Luminograph series (Imaging System for bio/chemiluminescence detection), etc. Their products have been used in many laboratories in Japan and other countries.
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