Ibidi - Labware - µ-Slide Spheroid Perfusion

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AUSTRALIA

Benoit Fissot, General Manager, Technology, Australia & New Zealand

Phone 1800 010 114 (hotline)

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DKSH Australia Pty Ltd
1 Beyer Road
3195 Braeside VIC
Australia

NEW ZEALAND

Benoit Fissot, General Manager, Technology, Australia & New Zealand

Phone 0800 951 010

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DKSH New Zealand Limited
33 Westpoint Drive
0618 Hobsonville, Auckland
New Zealand

VIETNAM

Nguyen Thi Xuan Mai, Senior Manager, National Sales

Phone +84 28 3812 5806 ext 89604

Fax +84 8 3812 5807

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DKSH Technology
5th Floor, Viettel Complex, 285 Cach Mang Thang Tam Ward 12, District 10/6th Floor, Peakview Tower, 36 Hoang Cau O Cho Dua Ward
70000 Ho Chi Minh City / Dong Da District, Ha Noi
Vietnam

Instructions µ-Slide Spheroid Perfusion - Protected
(PDF, 6 MB)

A perfusable channel µ-Slide with 3 x 7 wells for long-term spheroid cultivation and high-end microscopy

Product outline:

Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
For use with the ibidi Pump System or any other pump device with Luer connectors
Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
Spheroid generation directly in the wells
Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
Organ-on-a-chip setups with up- and downstream metabolization
Live cell imaging and microscopy of 3D cell aggregates
Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates

Technical specifications

Outer dimensions (w x l)	25.5 x 75.5 mm²
Number of channels	3
Number of wells	3 x 7
Volume per well	3.5 μl
Well height (bottom niche)	0.4 mm
Well height (total)	1.3 mm
Well diameter (bottom)	0.8 mm
Channel volume (total)	45 µl
Channel height	0.2 mm
Channel width	1.0 mm
Growth area per well	0.5 mm²
Coating area using 3.5 µl	9.7 mm²
Adapters	Female Luer
Volume per reservoir	60 µl

Slide comparison

	µ-Slide Spheroid Perfusion	µ-Slide I Luer 3D	µ-Slide I Luer
Application
Perfusion of samples	Yes	Yes	Yes
Defined shear stress on cell monolayers	No	Yes, on gel matrix	Yes, on coverslip
Gel matrices for 3D	No	Yes	No
Cell type
Spheroids/organoids	Yes, free floating in well	Yes, inside gel matrix only	No
Suspension cells	Yes, free floating in well	Yes, inside gel matrix only	Yes, in flow suspension only
Adherent cells	Yes, on coverslip	Yes, inside or on gel matrix	Yes, on coverslip

ibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.