Thermo Scientific™ - Microbiology - MicroSEQ & RapidFinder STEC PCR Assays

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features: 

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample 
• Genome sequencing using the Applied Biosystems SOLiD™ or 5500 Series Genetic Analyzer platforms helps ensure high specificity 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute and AFNOR according to ISO 16140 

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features: 

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample 
• Genome sequencing using the Applied Biosystems SOLiD™ or 5500 Series Genetic Analyzer platforms helps ensure high specificity 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute and AFNOR according to ISO 16140 

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features: 

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample 
• Genome sequencing using the Applied Biosystems SOLiD™ or 5500 Series Genetic Analyzer platforms helps ensure high specificity 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute and AFNOR according to ISO 16140 

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total) 
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control 

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total) 
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control 

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total) 
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples 
• Get optimal performance on a wide range of food sample and environmental surface types 
• Eliminate false negatives with internal positive control 

This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ® Listeria monocytogenes Detection Kit detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. 

• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total) 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample types 
• Eliminate false negatives with internal positive control 
• Validated by the AOAC Research Institute 

The MicroSEQ® Listeria monocytogenes Detection Kit Starter Kit is recommended for first time users in laboratories that routinely prepare up to 100 samples a day. It detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. 

• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total) 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• PrepSEQ™ Rapid Spin Sample Preparation Kit included
• Get optimal performance on a wide range of food sample types 
• Eliminate false negatives with internal positive control 
• Validated by the AOAC Research Institute 

This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ® Salmonella spp. Detection Kit detects Salmonella spp. simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. 

• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total) 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample types 
• Eliminate false negatives with internal positive control 
• Validated by the AOAC Research Institute 

The MicroSEQ Salmonella Species Detection Starter Kit with PrepSEQ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Salmonella species simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan probe to detect the amplified sequence. The MicroSEQ Salmonella Species Detection Kit is optimized for use with the PrepSEQ Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ SalmonellaSpecies Detection Kit with a 100 reaction PrepSEQ Nucleic Acid Extraction Kit and offer it here as a single product.

Key features

• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 19 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ pathogen detection kits
• Validated by the AOAC Research Institute

The MicroSEQ® Salmonella spp. Detection Kit Starter Kit with PrepSEQ™ RapidSpin Sample Preparation Kit – Extra Clean enables the detection of Salmonella spp. simply, reliably, and rapidly in food samples using the Applied Biosystems® 7500 Fast Real-Time PCR System. It is recommended for first time users in laboratories that routinely prepare up to 100 samples a day and prefer a spin column-based workflow. This starter kit combines the PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean with a MicroSEQ Salmonella spp. Detection Kit for ease of ordering. The benefits of this workflow include:

• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process 
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total) 
• Detect 1-3 colony-forming units (CFU) per 25 grams of food 
• Get optimal performance on a wide range of food sample types 
• Eliminate false negatives with internal positive control 
• Validated by the AOAC Research Institute 

The Applied Biosystems™ RapidFinder™ STEC Confirmation Assay is part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E. coli (STEC) O157:H7 and Big 6 non‐O157:H7 serogroup (O26, O45, O103, O111, O121, and O145) in a variety of beef products. Samples assessed as positive using the RapidFinder STEC Screening Assay are further tested using this RapidFinder STEC Confirmation Assay. All reactions include an internal positive control (IPC) and ROX™ passive reference.

The Applied Biosystems™ RapidFinder™ STEC Screening Assay is part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E. coli (STEC) O157:H7 and Big 6 non‐O157:H7 serogroup (O26, O45, O103, O111, O121 and O145) in a variety of beef products. The RapidFinder STEC Screening Assay was co-developed with the USDA-ARS and contains 96 complete qPCR assays targeted to screen for Shiga toxin 1 and 2 (stx1/stx2), eae, and potential O157:H7 presence. The assay is designed to be inclusive of all know allelic variants of stx and eae, including stx2g and stx2f. All reactions include an internal positive control (IPC) and ROX™ passive reference. Samples assessed as negative require no further testing. Samples assessed as positive must be further tested using the RapidFinder™ STEC Confirmation Assay.